التأثير التطفيري للمستخلص الكحولي لبذور نبات الحرمل في كونيدات الفطرAspergillus amstelodami

(1000 1500 2000 2500) / Aspergillus amstelodami pretreatment method growth mediated method . 8-Azaadenin. t 0.05 ( ) amstelodami A. .The mutagenicity effect of four sublethal concentrations of the alcoholic extract of Peganum harmala seeds including (1000, 1500, 2000, 2500) g/ml in the conidia of the fungus Aspergillus amstelodami was tested by using the two methods; pretreatment method and growth mediated method.The results of the two methods using the four studied concentrations showed a percentage of repeated mutagenic genes induced by the alcoholic extract and the resistance to 8-Azaadenin. Statistical analysis of the two methods, using t-test at a probability rate of 0.05, showed a significant difference between the mean mutation frequency induced by the extract compared with the zero treatment (spontaneous mutation) this result suggests amutagenic effect of the alcoholic extract of Peganum harmala seeds in the fungus A.amstelodami.