Evaluation of antibacterial effects of Eucalyptus camaldulensis L., extracts against some pathogenic bacteria in vitro and HPLC-DAD

Section: Article

Abstract

       Our study  was carried out in  laboratory of Alnoor university –Iraq  in ( 2025) to identify active compounds and evaluate  in vitro antimicrobial which  include   effects  of  hexane and aquatic  extracts with three concentration  on two species of bacteria , the  phenolic compounds for  Eucalyptus camaldulensis tree  identify  active compounds extract was  prepared by using Soxhlet  hexane and Aqueous as a solvent HPLC High performers liquid Chromatography , was used to diagnose and identify  phenolic compounds in  Eucalyptus extract. Results showed  identification of 5 active phenolic compounds, (Quercetin, Kaempferol, Ferulic, Gallic acid, Rutine).


These differences in chemical compounds could be used as an important indicator for plant identification.    


The second aspect of  study investigated  inhibitory effect of two organic solvents on two bacterial species (Staphylococcus aureus and Escherichia coli). Research revealed that  inhibition rates varied depending on solvent used. Furthermore, the in vitro antimicrobial potential of E. Camaldulensis extracts demonstrated varying degrees of inhibition at different concentrations, which also differed between  Gram-positive and negative bacteria.

References

  1. AL-CHAABAWI, H.M. and SATMBEKOVA, D.K., 2023. Obtaining and Research of methanolic extract from eucalyptus plant. Journal of Survey in Fisheries Sciences, vol. 10, no. 3S, pp. 3329-3336.
  2. Al-Jubouri, Sabiha Hussein Ahmed (2007). Physicochemical study of pentosanate extracts Al-Snafi AE. Traditional uses of Iraqi medicinal plants. IOSR J Pharm. 2018;8(8):32–96. [Google Scholar]
  3. Attallah NG, Negm WA, Elekhnawy E, Elmongy EI, Altwaijry N, El-Haroun H, El-Masry TA, El-Sherbeni SA. Elucidation of phytochemical content of Cupressus macrocarpa leaves: in vitro and in vivo antibacterial effect against methicillin-resistant Staphylococcus aureus clinical isolates. Antibiotics. 2021 Aug;10(8):890.
  4. Babayi, H.; Kolo, I.; Okogun, J.I. and Ijah, U.J.J. (2004).The Antimicrobial Activities of Methanolic Extracts of Eucalyptus camaldulensis and Terminalia catappaAgainst Some Pathogenic Microorganisms.Biokem., 16(2):106-111.
  5. Bachir, R.G.; Benali, M. Antibacterial Activity of the Essential Oils from the Leaves of Eucalyptus Globulus against Escherichia Coli and Staphylococcus aureus. Asian Pac. J. Trop. Biomed. 2012, 2, 739–742. [CrossRef
  6. Balogun M, et al. A review of Piper guineense (African Black Pepper) Ijppr n Huma 2016;6(1):368–384. [Google Scholar]
  7. Bauer, A.W., 1966. Antibiotic susceptibility testing by a standardized single disc method. Am. J. Clin. Patho., 45, 149-158.
  8. Benabdesslem, Y., Hachem, K., Me´barki, M., 2020. Chemical composition of the essen- tial oil from the leaves of Eucalyptus globulus Labill. Growing in southwest Algeria. Journal of Essential Oil-Bearing Plants 23, 1154–1160.
  9. Bernabé-Antonio, A., L. Álvarez, E. Salcedo-Pérez, F.L.D. Toral,J. Anzaldo-Hernándezand and F. Cruz-Sosa. 2015. Fatty acid profile of intact plants of two different sites and callus cultures derived from seed and leaf explants of CalophyllumbrasilienseCambess: A new resource of non- edible oil. Ind. Crops Prod., 77: 1014-1019
  10. Cheesbrough M. Medical Laboratory Manual for Tropical Countries. Oxford, United Kingdom: Oxford, Publishers; 1984. Microscopic examination of specimens; pp. 32–33. [Google Scholar]
  11. Cheesbrough M. Medical Laboratory Manual for Tropical Countries. Oxford, United Kingdom: Oxford, Publishers; 1984. Microscopic examination of specimens; pp. 32–33. [Google Scholar]
  12. Cruz MC, Santos PO, Barbosa Jr AM, de Mélo DL, Alviano CS, Antoniolli AR, Alviano DS, Trindade RC. Antifungal activity of Brazilian medicinal plants involved in popular treatment of mycoses. J Ethnopharmacol. 2007 May 4;111(2):409–412.
  13. D. R. Batish, H. P. Singh, N. Setia, S. Kaur and R. K. Kohli; Phytotoxicity.; 2006, 61, 465–470.
  14. D. W. Reische, D. A. Lillard and R. R. Eitenmiller; Antioxidants in food lipids. In Chemistry Nutrition and Biotechnology; Ahoh, C.C., Min, D.B., Eds.; Marcel Dekker: New York, NY, USA, 1998; Volume 1, pp. 423-448.
  15. Dahiya P, Purkayastha S. Phytochemical Screening and Antimicrobial Activity of Some Medicinal Plants Against Multi-drug Resistant Bacteria from Clinical Isolates. Indian J Pharm Sci. 2012 Sep;74(5):443-50. doi: 10.4103/0250-474X.108420. Erratum in: Indian J Pharm Sci. 2012 Nov;74(6):563.
  16. Elansary, H.O., Salem, M.Z.M., Ashmawy, N.A., Yessoufou, K., El-Settawy, A.A.A., 2017. In vitro antibacterial, antifungal and antioxidant activities of Eucalyptus spp. leaf extracts related to phenolic composition. Natural Product Research 31, 2927–2930.
  17. Famurewa A, et al. Abrogation of hepatic damage induced by anticancer drug methotrexate by Zobo (Hibiscus sabdariffa extract) supplementation via targeting oxidative hepatotoxicity in rats. Journal of Dietary Supplements. 2018;10:1456502. doi: 10.1080/19390211.2018.1456502. [DOI] [PubMed] [Google Scholar
  18. G. Sacchetti, S. Maietti, M. Muzzoli, M. Scaglianti, S. Manfredini, M. Radice and R. Bruni; Comparative evaluation of 11 essential oils of different origin as functional antioxidants antiradicals and antimicrobials in foods; Food Chem.; 2005, 91, 621–632.
  19. Ghaffar, A., M. Yameen, S. Kiran, S. Kamal, F. Jalal, B. Munir and A. Jabbar. 2015. Chemical composition and In vitro evaluation of the antimicrobial and antioxidant activities of essential oils extracted from seven Eucalyptus species. Molecules, 20(11): 20487-20498.
  20. Ivanov, M., Kannan, A., Stojkovic, D.S., Glamoclija, J., Calhelha, R.C., Ferreira, I.C.F.R., Sanglard, D., Sokovic, M., 2021. Camphor and eucalyptol-anticandidal spectrum, antivirulence effect, efflux pumps interference and cytotoxicity. International Jour- nal of Molecular Sciences 22, 1–14.
  21. J. A. Duke; The Green Pharmacy; Rodale Press: Emmaus, PA, USA, 1997; pp. 140–255.
  22. J. S. Hur, S.Y. Ahn, Y. J. Koh and C. Lee; Antimicrobial properties of cold-tolerant Eucalyptus species against phytopathogenic fungi and food-borne bacterial pathogens; Plant Pathol. J.; 2000, 16, 286-289.
  23. K. Husnu, C. Baser and F. Demirci; Chemistry of essential oils; Flavour Fragr. J.; 2007, 5, 43–86.
  24. K.P. Svoboda and S.G. Deans; Biological activities of essential oils from selected aromatic plants; Acta Hortic.; 1998, 39, 203–209.
  25. Laghari, M.H., J. Mangi, M. Gorar, S. Sohu, A. Hayat, S. Zahid, F.A. Khatri, K.A. Gishkori, M.Y. Seelro, A. Qureshi, S.A. Soomro and A.R. Sidhu. 2023. Identification and quantification of phenolic compounds by HPLC-DAD and antioxidant activity from Cordia gharaf Plant. J. Appl. Res. Plant Sci., 4(02): 653-661.
  26. Luis, A., N. Gil, M.E. Amaral and A.P. Duarte. 2012. Antioxidant activities of extracts from Acacia melanoxylon, Acacia dealbata and Olea europaea and alkaloids estimation. Int. J. Pharm. Pharm. Sci., 4(1): 225-231
  27. M. Ekor; The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety.; Front Pharmacol.; 2014, 4,177.
  28. M. H. H. Roby, M. A. Sarhan, K. A. H. Selim and K.I. Khalel; Evaluation of antioxidant activity total phenols and phenolic compounds in thyme (Thymus vulgaris L.) sage (Salvia officinalis L.) and marjoram (Origanum majorana L.) extracts; Ind. Crops Prod.; 2002, 43, 827–831.
  29. Maurya, A. and S.K. Srivastava. 2012. Determination of ursolic acid and ursolic acid lactone in the leaves of Eucalyptus tereticornis by HPLC. J. Braz. Chem. Soc., 23: 468-472
  30. Medicinal plants have a long history in the treatment of vari- ous ailments and diseases especially infectious diseases due to their therapeutic benefits (Saad et al. 2017) Mossalem
  31. Memon, A.A., N. Memon, D.L. Luthria, M.I. Bhanger and A.A. Pitafi. 2010. Phenolic acids profiling and antioxidant potential of mulberry (Morus laevigata W., Morus nigra L., Morus alba L.) leaves and fruits grown in Pakistan. Pol. J. Food Nutr. Sci., 60(1): 25-32.
  32. Memon, S., A.A. Chandio, A.A. Memon, Q.K. Panhwar, S.M. Nizamani, A.A. Bhatti and N.A. Brohi. 2017. Synthesis, characterization, and exploration of antimicrobial activity of copper complex of diamide derivative of p-tert-butylcalix arene. Polycycl. Aromat. Compd., 37(5): 362-374.
  33. Memon, S., A.A. Chandio, A.A. Memon, Q.K. Panhwar, S.M. Nizamani, A.A. Bhatti and N.A. Brohi. 2017. Synthesis, characterization, and exploration of antimicrobial activity of copper complex of diamide derivative of p-tert-butylcalix arene. Polycycl. Aromat. Compd., 37(5): 362-374.
  34. Nascimento, Gislene G. F.; Locatelli, Juliana; Freitas, Paulo C.; Silva, Giuliana L. (2000). Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria. Brazilian Journal of Microbiology, 31(4), –. doi:10.1590/S1517-83822000000400003
  35. P. J. Quinn, M. E. Carter, B. Markey and G. R. Carter; Antimicrobial activity of plant extracts.; Clin. Vet. Microb.; 2004, 7, 543–549.
  36. Pavithra, P.S., N. Sreevidya and R.S. Verma. 2009. Antibacterial activity and chemical composition of essential oil of Pamburus missionis. J. Ethnopharm., 124(1): 151-153
  37. Peirano G: Multi resistant Enterobacteriaceae new threat to an old prob; expect review of anti-infective therapy. Expert Rev Anti Infect Ther 6, 657–669 (2008)
  38. Rahmani, R., Jouaidi, M., Bouajila, J., Debouba, M., 2020. African mustard (Brassica tour- nefortii) as source of nutrients and nutraceuticals properties. Journal of Food Sci- ence 85, 1856–1871.
  39. Sabo, A.V., Knezevic, P., 2019. Antimicrobial activity of Eucalyptus camaldulensis Dehn. Plant extracts and essential oils: a review. Industrial Crops and Products 132, 413– 429.
  40. Sharifi-Rad, J., Sureda, A., Tenore, G.C., Daglia, M., Sharifi-Rad, M., Valussi, M., Tundis, R., Sharifi-Rad, M., Loizzo, M.R., Ademiluyi, A.O., Sharifi-Rad, R., Ayatollahi, S.A., Iriti, M., 2017. Biological activities of essential oils: from plant chemoecology to traditional healing systems. Molecules 22, 70.
  41. Sharma A, Verma R, Ramteke P. Antibacterial activity of some medicinal plants used by tribals against UTI causing pathogens. World Appl Sci J. 2009;7:332–9.
  42. Sharmeen, J.B., Mahomoodally, F.M., Zengin, G., Maggi, F., 2021. Essential oils as natural sources of fragrance compounds for cosmetics and cosmeceuticals. Molecules 26, 666.
  43. Siddiqui, M.S., A.A. Memon, S. Memon and S.G. Baloch. 2017. Cuscutareflexa as a rich source of bioactive phenolic compounds. J. Herbs Spices Med. Plants., 23(2): 157-168.
  44. Sidhu, A.R., A. Basit, A. Hayat, S., Mangrio, S. Arain, T. Khalid, H.I. Mohamed and A. Elhakem. 2022. Quality characteristics, phytochemical analysis, and antioxidant of extract Cuscuta reflexa (Roxb.). Not. Bot. Hort.Agrobot. Cluj-Napoca., 50(3): 12691-12691
  45. Sidhu, A.R., A. Basit, A. Hayat, S., Mangrio, S. Arain, T. Khalid, H.I. Mohamed and A. Elhakem. 2022. Quality characteristics, phytochemical analysis, and antioxidant of extract Cuscuta reflexa (Roxb.). Not. Bot. Hort.Agrobot. Cluj-Napoca., 50(3): 12691-12691.
  46. Sulaiman, C.T., V.K. Gopalakrishnanand and I. Balachandran. 2014. Phenolic compounds and antioxidant properties of selected Acacia species. J. Biol. Act. Prod. Nat., 4(4): 316-324.
  47. The aqueous extract produced from kinos of Eucalyptus spp. were given to treat infections of the wounds and eyes -17)(( von Martius , et al , 2012)
  48. Von Martius, S.; Hammer, K.A, and Locher, C. (2012). Chemical Characteristics and Antimicrobial Effects of Some Eucalyptus kinos. J. Ethnopharmacol., 144(2):293-9.
  49. W. Wang, N. Wu, Y. G. Zu and Y. J. Fu; Antioxidative activity of Rosmarinus oficinalis L. essential oil compared to its main components; Food Chem.; 2008, 108(3), 1019–1022.
  50. Wigmore, S.M; Naiker, M. and Bean, D.C. (2016). Antimicrobial Activity of Extracts from Native Plants of Temperate Australia. Pharmacog. Commn., 6(2): 80-84.
  51. Yadav, R.N.S. and M. Agarwala. 2011. Phytochemical analysis of some medicinal plants. J. Phytol., 3(12): 10-14.
  52. Yik Ling Chew, Elaine Wan Ling Chan, Pei Ling Tan, Yau Yan Lim, Johnson Stanslas, Joo Kheng Goh. Assessment of phytochemical content, polyphenolic composition, antioxidant and antibacterial activities of Leguminosae medicinal plants in Peninsular Malaysia. BMC Complementary and Alternative Medicine 2011; 11: 1-10.
Download this PDF file

Statistics

How to Cite

Evaluation of antibacterial effects of Eucalyptus camaldulensis L., extracts against some pathogenic bacteria in vitro and HPLC-DAD. (2026). College of Basic Education Research Journal, 22(2), 223-242. https://doi.org/10.33899/berj.2026.Vol22.Iss2.61370
Copyright and Licensing

How to Cite

Evaluation of antibacterial effects of Eucalyptus camaldulensis L., extracts against some pathogenic bacteria in vitro and HPLC-DAD. (2026). College of Basic Education Research Journal, 22(2), 223-242. https://doi.org/10.33899/berj.2026.Vol22.Iss2.61370