Molecular Diagnosis of Enterobacter Cloacae and Stutzerimonas Stutzeri and Study of Effect of Some Plant Extract
Abstract
Periodontal pockets are thought to provide spawning grounds for a variety of microorganisms. Emergence of antibiotic-resistant bacterial strains poses a risk on course of treatment and may require surgical removal of gum pocket. This study's aim was to determine the bacteria found in gum pockets using PCR for 16S rRNA gene. As well as impact of plant extracts on growth of these strains. Five new bacterial strains have been registered at NCBI, including Stutzerimonas stutzeri, Staphylococcus aureus, and Enterobacter cloacae. All identified strains were susceptible to Augmentin and Ciprofloxacin but resistant to Cephalothin. Cefixime and Bacitracin were not effective against 90% of strains. Amoxicillin resistance rate was 50%. All strains were resistant to Turmeric and Oregano extracts at concentrations of 100 and 200 mg/ml, with exception of Staph. aureus FKZEW99, which was susceptible to Oregano at 200 mg/ml. Identified strains' responses to clove extract differed in terms of sensitivity and resistance. The overuse of antibiotics leads to appearance of new bacterial strains resistant to a range of treatments.
References
- Abdulrazzaq, R., & Faisal, R. (2022). Efficiency of hichrome Enterococcus faecium agar in the isolation of Enterococcus spp. and other associated bacterial genera from water. Journal of Life and Bio Sciences Research, 3(01), 01-06.
- Al-Noamy, N. A. (2020). Detection of the inhibitory effect of the leaves, seed and fruits of Cydonia oblonga on some gram positive and negative bacteria. Rafidain J. Sci, 29, 10-19.
- Al-saidy, H. A., Al-qrtani, Y. M., & Al-Zubaidy, N. A. (2013). Effect of Crued Extracts for Cinnamum (Cinnamum zeylaicum), Syzygium (Syzygium aromaticum) and Thymus (Thymus vulgaris) on Growth of Aspergillus flavus which produce Aflatoxin B1. Diyala Agricultural Sciences Journal, 5(2), 593-602.
- Bottery, M. J., Matthews, J. L., Wood, A. J., Johansen, H. K., Pitchford, J. W., & Friman, V. P. (2022). Inter-species interactions alter antibiotic efficacy in bacterial communities. The ISME journal, 16(3), 812-821.
- Cappuyns, I., Cionca, N., Wick, P., Giannopoulou, C., & Mombelli, A. (2012). Treatment of residual pockets with photodynamic therapy, diode laser, or deep scaling. A randomized, split-mouth controlled clinical trial. Lasers in medical science, 27, 979-986.
- Colombo, A. P. V., do Souto, R. M., Araújo, L. L., Espíndola, L. C. P., Hartenbach, F. A. R., Magalhães, C. B., ... & da Silva-Boghossian, C. M. (2023). Antimicrobial resistance and virulence of subgingival staphylococci isolated from periodontal health and diseases. Scientific Reports, 13(1), 11613.
- Cuesta, A. I., Jewtuchowicz, V., Brusca, M. I., Nastri, M. L., & Rosa, A. C. (2010). Prevalence of Staphylococcus spp and Candida spp in the oral cavity and periodontal pockets of periodontal disease patients. Acta Odontológica Latinoamericana, 23(1), 20-26.
- Drisko, C. H., & Lewis, L. H. (1996). Ultrasonic instruments and antimicrobial agents in supportive periodontal treatment and retreatment of recurrent or refractory periodontitis. Periodontology 2000, 12(1), 90-115.
- Fatimah, B., Kinasih, D. S. S., Retnowati, W., & Setiawati, Y. (2022). Antibacterial Activity Test of Turmeric Extract (Curcuma longa) From Madura Island Against Staphylococcus aureus. Jurnal Kesehatan Prima, 16(1), 74-82.
- Faujdar, S. S., Bisht, D., & Sharma, A. (2020). Antibacterial activity of Syzygium aromaticum (clove) against uropathogens producing ESBL, MBL, and AmpC beta-lactamase: Are we close to getting a new antibacterial agent?. Journal of family medicine and primary care, 9(1), 180-186.
- Gamboa, F., García, D. A., Acosta, A., Mizrahi, D., Paz, A., Martínez, D., ... & Abba, M. (2013). Presence and antimicrobial profile of gram-negative facultative anaerobe rods in patients with chronic periodontitis and gingivitis. Acta Odontológica Latinoamericana, 26(1), 24-30.
- Giannopoulou, C., Cappuyns, I., Cancela, J., Cionca, N., & Mombelli, A. (2012). Effect of photodynamic therapy, diode laser, and deep scaling on cytokine and acute‐phase protein levels in gingival crevicular fluid of residual periodontal pockets. Journal of periodontology, 83(8), 1018-1027.
- Han, J., Wang, P., & Ge, S. (2016). The microbial community shifts of subgingival plaque in patients with generalized aggressive periodontitis following non-surgical periodontal therapy: a pilot study. Oncotarget, 8(6), 10609.
- Hao, Y., Li, J., & Shi, L. (2021). A carvacrol-rich essential oil extracted from oregano (Origanum vulgare “Hot & Spicy”) exerts potent antibacterial effects against Staphylococcus aureus. Frontiers in Microbiology, 12, 741861.
- Haque, M. M., Yerex, K., Kelekis-Cholakis, A., & Duan, K. (2022). Advances in novel therapeutic approaches for periodontal diseases. BMC oral health, 22(1), 492.
- Hassan, M. A., Abd El-Aziz, S., Elbadry, H. M., Samy, A., & Tamer, T. M. (2022). Prevalence, antimicrobial resistance profile, and characterization of multi-drug resistant bacteria from various infected wounds in North Egypt. Saudi Journal of Biological Sciences, 29(4), 2978-2988.
- Hu, K., Zeng, L., Zhang, J., Li, H., Su, S., Zhao, Y., ... & Zhang, X. (2020). Antibiotic susceptibility and molecular analyses of clinical Enterobacter cloacae isolates in Eastern Heilongjiang Province, China. Annals of Palliative Medicine, 9(3), 1211219-1211219.
- Ilham, L. A., Herla, R., Dwi, S., & DewiRestuana, S. (2018). Antimicrobial activity of turmeric leaf extract against Escherichia coli, Staphylococcus aureus, Shigella dysenteriae, and Lactobacillus acidophilus. In IOP Conference Series: Earth and Environmental Science (Vol. 205, No. 1, p. 012048). IOP Publishing.
- Jepsen, K., Falk, W., Brune, F., Cosgarea, R., Fimmers, R., Bekeredjian-Ding, I., & Jepsen, S. (2022). Prevalence and antibiotic susceptibility trends of selected enterobacteriaceae, enterococci, and candida albicans in the subgingival microbiota of German periodontitis patients: a retrospective surveillance study. Antibiotics, 11(3), 385.
- Kulik, E. M., Lenkeit, K., Chenaux, S., & Meyer, J. (2008). Antimicrobial susceptibility of periodontopathogenic bacteria. Journal of antimicrobial chemotherapy, 61(5), 1087-1091.
- Mombelli, A. (2018). Microbial colonization of the periodontal pocket and its significance for periodontal therapy. Periodontology 2000, 76(1), 85-96.
- Montagna, M. T., Triggiano, F., Barbuti, G., Bartolomeo, N., De Giglio, O., Diella, G., ... & Caggiano, G. (2019). Study on the in vitro activity of five disinfectants against nosocomial bacteria. International journal of environmental research and public health, 16(11), 1895.
- Nostro, A., Roccaro, A. S., Bisignano, G., Marino, A., Cannatelli, M. A., Pizzimenti, F. C., ... & Blanco, A. R. (2007). Effects of oregano, carvacrol and thymol on Staphylococcus aureus and Staphylococcus epidermidis biofilms. Journal of medical microbiology, 56(4), 519-523.
- Peterson, C. T., Vaughn, A. R., Sharma, V., Chopra, D., Mills, P. J., Peterson, S. N., & Sivamani, R. K. (2018). Effects of turmeric and curcumin dietary supplementation on human gut microbiota: A double-blind, randomized, placebo-controlled pilot study.
- Popova, C., Dosseva-Panova, V., & Panov, V. (2013). Microbiology of periodontal diseases. A review. Biotechnology & Biotechnological Equipment, 27(3), 3754-3759.
- Shi, M., Wei, Y., Hu, W., Nie, Y., Wu, X., & Lu, R. (2018). The subgingival microbiome of periodontal pockets with different probing depths in chronic and aggressive periodontitis: a pilot study. Frontiers in cellular and infection microbiology, 8, 124.
- Suzuki, N., Yoneda, M., & Hirofuji, T. (2013). Mixed red‐complex bacterial infection in periodontitis. International journal of dentistry, 2013(1), 587279.
- Tamura, K., Stecher, G., & Kumar, S. (2021). MEGA11: molecular evolutionary genetics analysis version 11. Molecular biology and evolution, 38(7), 3022-3027.
- Wang, Z., Sun, X., Chen, X., Wang, H., & He, H. (2023). The Mass Spectrometry Identification, Antimicrobial Genes Detection, and Proteomics Analysis of Stutzerimonas stutzeri Strain Was Isolated from Industrial Wastewater. Separations, 10(9), 461.
- Yoo, J. H., Baek, K. H., Heo, Y. S., Yong, H. I., & Jo, C. (2021). Synergistic bactericidal effect of clove oil and encapsulated atmospheric pressure plasma against Escherichia coli O157: H7 and Staphylococcus aureus and its mechanism of action. Food Microbiology, 93, 103611.
Download this PDF file
Statistics
How to Cite
Copyright and Licensing

This work is licensed under a Creative Commons Attribution 4.0 International License.







