Evaluating Effect of Aqueous Extract of Seeds of Raphanus Sativus on Histological Changes in Kidney Resulting from Complications of Diabetes in Rats

Section: Article
Issue
Vol. 21 No. 4 (2025): Volume 21 Issues 4
Published
Dec 1, 2025
Pages
286-304

Abstract

Study aimed to know effect of  aqueous extract of radish seeds and its role in reducing and maintaining  levels of glucose, insulin hormone, and  effect on kidney functions represented by levels of creatinine and urea in  blood, as well as its role in alleviating tissue changes in  kidneys. Twenty-four rats were used and divided into three equal groups: first (non-diabetic), second group, control group, in which experimental diabetes was induced with streptozotocin at a dose of 50 mg/kg, and third group, diabetic, treated with  aqueous extract at a dose of 300 mg/kg for two periods (three and six weeks). After that, blood samples were collected to measure levels of glucose, insulin hormone, urea and creatinine. Tissue changes in  kidneys were also examined. Results showed a significant difference in glucose levels for diabetic control group after injection with STZ and a significant decrease in  aqueous radish group after six weeks compared to period after STZ injection. Results also indicate a significant decrease in concentration of Insulin hormone after induction compared to healthy control group. After six weeks of treatment with extract, an increase in level of insulin hormone was observed compared to period after injection. Results also indicate an increase in level of urea and creatine in blood serum after inducing diabetes. After a period of treatment with this extract, a decrease in level of urea and creatine was observed compared to period after injection.

References

  1. Alipour, M., Malihi, R., Hosseini, S. A., Abbasnezhad, A., Ghavami, A., Shahmohammadi, H. A., & Ghanavati, M. (2018). The effects of catechins on related risk factors with type 2 diabetes: a review Progress in Nutrition, 20(1), 12-20.‏
  2. Aryal, D., Joshi, S., Thapa, N. K., Chaudhary, P., Basaula, S., Joshi, U., ... & Parajuli, N. (2024). Dietary phenolic compounds as promising therapeutic agents for diabetes and its complications: A comprehensive review. Food Science & Nutrition.‏
  3. Bamanikar, S. A., Bamanikar, A. A., & Arora, A. (2016). Study of Serum urea and Creatinine in Diabetic and nondiabetic patients in a tertiary teaching hospital. The Journal of Medical Research, 2(1),12-15.
  4. Banihani, S. A. (2017). Radish (Raphanus sativus) and diabetes. Nutrients, 9(9), 1014.‏
  5. Blair , M. (2016). Diabetes mellitus review. Urologic nursing, 36(1) .
  6. Fajarwati, I., Solihin, D. D., Wresdiyati, T., & Batubara, I. (2023, May) Administration of alloxan and streptozotocin in Sprague Dawley rats and the challenges in producing diabetes model. In IOP Conference Series: Earth and Environmental Science (Vol. 1174, No. 1, p. 012035). IOP Publishing.‏
  7. Furman, B. L. (2021). Streptozotocin‐induced diabetic models in mice and rats. Current protocols, 1(4), e78
  8. Goyal, S.N., Reddy, N.M., Patil, K.R., Nakhate, K.T., Ojha, S., Patil, C.R. &Agrawal, Y.O.( 2016). Challenges and issues with streptozotocin-induced diabetes-a clinically relevant animal model to understand the diabetes pathogenesis and evaluate therapeutics. Chemico-biological interactions, 244, 49-63.
  9. Jaafar, N. A., Ahmed, A. S., & Al-Sandooq, D. L. (2020). Detection of active compounds in radish Raphanus Sativus L. and their various biological effects. Plant Arch, 20(2), 1647-50.‏
  10. Kim, D. H., Lim, S. H., & Lee, J. Y. (2023). Expression of RsPORB Is Associated with Radish Root Color. Plants, 12(11), 2214.
  11. Le Grand, A., Wondergem, P. A., Verpoorte, R., & Pousset, J. L. (1988) Anti-infectious phytotherapies of the tree-savannah of Senegal (West-Africa) II. Antimicrobial activity of 33 species. Journal of ethnopharmacology, 22(1), 25-31.
  12. Mohamed, S. M., Aly, T. A., Khattab, M. S., Abdel-Rahim, E. A., & Al-Farga, A. (2023). Pathological and biochemical evaluation of radish microgreen on diabetes and aflatoxicosis in rats. Environmental Science and Pollution Research, 30(43), 98389-98399.‏
  13. Moram, G. S. E., Kholief, T. E. S., & Ahmed, A. T. F. (2015). Antioxidant effect of radish (Raphanus sativus L.) and leek (Allium porrum L.) juices against hepatotoxicity and nephrotoxicity induced by dimethoate in male albino mice. World Journal of Pharmaceutical Research, 4(12), 215-46.
  14. Nishio, T., & Kitashiba, H. (Eds.). (2017). The radish genome. Springer .
  15. Pandya, D., Nagrajappa, A. K., & Ravi, K. S. (2016). Assessment and correlation of urea and creatinine levels in saliva and serum of patients with chronic kidney disease, diabetes and hypertension-a research study. Journal of clinical and diagnostic research : JCDR, 10(10), ZC58.
  16. Parasuraman, S., Balamurugan, S., Christapher, P.V., Petchi, R.R., Yeng, W.Y., Sujithra, J. and Vijaya, C.( 2015). Evaluation of antidiabetic and antihyperlipidemic effects of hydroalcoholic extract of leaves of Ocimum tenuiflorum (Lamiaceae) and prediction of biological activity of its phytoconstituents. Pharmacognosy research, 7(2), p.156.
  17. Rios, J. L., Recio, M. C., & Villar, A. (1987). Antimicrobial activity of selected plants employed in the Spanish Mediterranean area. Journal of ethnopharmacology, 21(2), 139-152
  18. Satari, A. F., Srinivasa, V., Devaraju, M. S., & Ganapathi, M. (2020). Study on growth and root yield of radish (Raphanus sativus Lam.) aInfluenced by nutrition. Int. J. Curr. Microbiol. App. Sci, 9(8), 2466-2471.
  19. Shahbazian, H., & Rezaii, I. (2013). Diabetic kidney disease; review of the current knowledge. Journal of renal injury prevention, 2(2), 73.
  20. Sharma, M., Chan, H. K., Lavilla Jr, C. A., Uy, M. M., Froemming, G. R. A., & Okechukwu, P. N. (2023). Induction of a single dose of streptozotocin (50 mg) in rat model causes insulin resistance with type 2 diabetes mellitus. Fundamental & Clinical Pharmacology, 37(4), 769-778.‏
  21. Shukla, S., Chatterji, S., Mehta, S., Rai, P. K., Singh, R. K., Yadav, D. K., & Watal, G. (2011). Antidiabetic effect of Raphanus sativus root juice. Pharmaceutical Biology, 49(1), 32-37.‏
  22. Singh, B., Kumar, A., Singh, H., Kaur, S., Arora, S., & Singh, B. (2022) Protective effect of vanillic acid against diabetes and diabetic nephropathy by attenuating oxidative stress and upregulation of NF-kB, TNF-a and COX-2 proteins in rats. Phytotherapy Research, 36(3), 1338-1352.
  23. Ulvi, H., Yoldas, T., Müngen, B. and Yigiter, R.(2002). Continuous infusion of midazolam in the treatment of refractory generalized convulsive status epilepticus. Neurological Sciences, 23, pp.177-182.
  24. Zrouri, H., Elbouzidi, A., Bouhrim, M., Bencheikh, N., Kharchoufa, L., Ouahhoud, S., ... & Choukri, M. (2021). Phytochemical analysis, antioxidant activity, and nephroprotective effect of the Raphanus sativus aqueous extract Mediterranean Journal of Chemistry, 11(1), 84-94.
  25. DHAHIR, M. R., & AL-NAELY, A. J. (2022). Effect of alcoholic and nano-extract of red radish, Raphanus sativua on liver and kidney function in rats induced jaundiced. Iranian Journal of Ichthyology, 9, 148-160.‏
  26. Aly, T. A., Fayed Attia Koutb, F. M., Fayed, S. A., Ahmed, A. M., & ELRahim, E. A. (2020). Biochemical and histopathological evaluation of radish microgreen and clover etiolated sprouts against diabetic mellitus rats. European J Pharm Med Res, 7(2), 126-134.
  27. Kamil, A. M., Hussain, M., Kadoori, Y. T., & Ahmad, S. J. (2018). Eruca sativa and Raphanus sativus Oils Enhance Hepatic and Renal Tissues Regeneration in White Mice. Al-Mustansiriyah Journal of Science, 29(4).‏
  28. Abdullah, M. F. A. A. A., & Haamed, N. N. (2018). Effect of the salinity of irrigation water and spraying with selenium in the yield indicators and the qualitative traits for two cultivars of the okra plant (Abelmoschus esculentus L.) cultivated in greenhouses. Euphrates Journal of Agriculture Science, 10(2), 295-306.

Authors

Noura Salman Mohmmed

University of Mosul, College of Education for Girls, Department of Life Sciences, Mosul, Iraq.

Ayad Chachan Khorsheed

University of Mosul, College of Education for Girls, Department of Life Sciences, Mosul, Iraq.

Liqa Hussein Ali

University of Mosul, College of Education for Girls, Department of Life Sciences, Mosul, Iraq.

Identifiers

https://doi.org/10.33899/berj.2025.Vol21.Iss4.55910
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How to Cite

نوره سلمان محمد, اياد جاجان الداؤدي, & لقاء حسين علي الدليمي. (2025). Evaluating Effect of Aqueous Extract of Seeds of Raphanus Sativus on Histological Changes in Kidney Resulting from Complications of Diabetes in Rats. College of Basic Education Research Journal, 21(4), 286–304. https://doi.org/10.33899/berj.2025.Vol21.Iss4.55910
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